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Order of Screening Events
After your application has been submitted, you will be contacted within two weeks on the status of your application. The most critical factor for approval is optimization of your screen in a 384-well format with positive and negative controls (data submitted with your application). DsRNA controls can be generated using these protocols, and control siRNAs can be ordered from a number of vendors (i.e Ambion, Dharmacon, Qiagen, Invitrogen etc).
2. After approval of your application, please sign the data sharing agreement and upon receipt, we will contact you to schedule your screen. Unfortunately, at this time, we cannot assist in temporary housing arrangements. Once you have arrived at NYU, the screening staff will review your screening protocol and train you on relevant equipment.
3. The screener will be given a single test plate to ensure optimization of the screen.
here to view the test plate template.
Please note that unless indicated, wells contain 5ul of dsRNAs/siRNAs.
4. Data from the test plate will be reviewed by the RNAi Core Facility staff. If results confirm the feasibility of the screen, then the screener may start a pilot screen which consists of six 384-well plates (the first three screening plates in duplicate) from our library collection. Please see library description for the plate layout for the genomes.
5. After data from the pilot screen is entered into the RNAi Core database, the screener will meet with Assistant Director, Chi Yun, to receive approval to continue onto the rest of genome screen.
Before receiving siRNAs for secondary screening, screeners are required to submit their full protocol, assay conditions, results, hit criteria, and lists of hits.
Plate reader based screens take about 1 month to complete.
Visual screens may take 6 weeks to 2 months to complete.
If the test plate or pilot screen yield unsatisfactory results, the screener must return to his/her home institution for further assay optimization. If a screen is not performed, the screener will be charged for instrumentation usage. Click here for costs.
Instructions for formatting and uploading data will be provided after your pilot screen is completed. Once data is entered, instructions will be provided as how to view data and submit wells of interest and hits.